| U S A RECYCLING LLCFort Myers, FL | 1077166 | 2 |
| UFC TRANSPORT LLCFort Walton Beach, FL | 2014133 | 1 |
| UHBK TRUCKING LLCSebring, FL | 4504658 | 1 |
| ULTIMATE COACHES LLCOrlando, FL | 4166059 | 1 |
| UMBRELLA BUSES INCOrlando, FL | 3309402 | 15 |
| UNCLE TRUCKING LIMITED LIABILITY COMPANYDavenport, FL | 2376173 | 3 |
| UNDEFEATED TRUCKING LLCPolk City, FL | 3376324 | 1 |
| UNICAR TRANSPORT COMPANY CORPMedley, FL | 2529859 | 2 |
| UNIFIED EXPRESS LLCJacksonville, FL | 3501059 | 16 |
| UNION SERVICES CORPMiami, FL | 2840020 | 10 |
| UNIQUE SUPPLIES CORPORATIONFort Pierce, FL | 1530025 | 1 |
| UNIQUE VAN LINES INCMiami, FL | 1951425 | 1 |
| UNITED AUTO SOLUTIONS LLCHomestead, FL | 4278504 | 2 |
| UNITED RR XPRESS CORPWest Palm Beach, FL | 1012901 | 1 |
| UNITED TRANSPORT SOLUTIONS LLCPalm Coast, FL | 4077036 | 1 |
| UNITED WORLD TRANSPORTATION AND LOGISTICS LLCHialeah, FL | 4527055 | 1 |
| UNIVERSAL CARGO TRUCKING LLCMiami, FL | 4393330 | 1 |
| UNIVERSAL DIRECT TRANSPORTATION LLCTampa, FL | 232587 | 57 |
| UNIVERSAL PETROLEUM TANK SERVICE INCJacksonville, FL | 132625 | 1 |
| UNLIMITED AUTO CARRIER INCMiami, FL | 4116909 | 7 |
| UNLIMITED MOVING LLCTampa, FL | 2119975 | 3 |
| UPBEAT TRANSPORT SERVICE INCHoliday, FL | 2191374 | 2 |
| UPGRADE TRANSPORT INCDelray Beach, FL | 3016919 | 1 |
| UPLIFT ABILITIES INCPalm Bay, FL | 4087439 | 1 |
| UPSTAR LOGISTICS LLCMiami, FL | 4277229 | 20 |
| URBAN CONTRACTING GROUP I INCPompano Beach, FL | 3980264 | 3 |
| URBAN METALS LLCTampa, FL | 3638529 | 2 |
| UREMI TRANSPORTATION LLCPanama City Beach, FL | 3997655 | 3 |
| URIEL TRANSPORT SERVICES CORPOrlando, FL | 3587545 | 1 |
| URRA TRANSPORTATION SERVICES LLCLehigh Acres, FL | 3923098 | 2 |
| US 1 AUTO TRANSPORT INCLehigh Acres, FL | 4042311 | 2 |
| US 1 LOGISTICS LLCPonte Vedra, FL | 1348167 | 389 |
| US MOVING & VAN LINE LLCSunrise, FL | 1697411 | 1 |
| US NORTH FLORIDA TRANSPORT INCOrlando, FL | 2912996 | 1 |
| US SOUTH FLORIDA TRANSPORT LLCOpa Locka, FL | 2872474 | 1 |
| US TRANSCARGO INCMiami, FL | 3280524 | 1 |
| US TRANSPORTERS LLCOcala, FL | 3882232 | 1 |
| USA BOATHAULING INCHialeah, FL | 3945983 | 1 |
| USA TRANSPORTATION CORPLive Oak, FL | 2535562 | 1 |
| USA TRUCKING SERVICES LLCMiami, FL | 4396972 | 6 |
| USBR GENERAL SERVICES LLCTampa, FL | 3869710 | 5 |
| USG INTERNATIONAL LTDMiami, FL | 209460 | 4 |
| USHR LLCBunnell, FL | 2537137 | 32 |
| USMAN EXPRESS LLCPalm Coast, FL | 2296068 | 2 |
| USTA INCWinter Garden, FL | 3727556 | 2 |