| O & E INTERNATIONAL TRANSPORT INCLehigh Acres, FL | 3734024 | 6 |
| O & J SPEEDY TRANSPORT INCWest Palm Bch, FL | 3981497 | 4 |
| O & J TRANSPORTATION EXPRESS CORPHialeah, FL | 4128770 | 1 |
| O & S TRANSPORT LOGISTIC SERVICES LLCHialeah, FL | 4391118 | 1 |
| O D EXPRESS TRANSPORT LLCHialeah, FL | 1857398 | 1 |
| O R HUMPHREY LLCWauchula, FL | 572095 | 1 |
| O&L TRANSPORT LLCRiverview, FL | 3382785 | 2 |
| OAB TRANSPORT LLCPort Richey, FL | 3410097 | 2 |
| OAKLEY TRANSPORT INCLake Wales, FL | 281321 | 651 |
| OASIS LOGISTICS GROUP LLCDavenport, FL | 4579027 | 2 |
| OBC STAR TRANSPORT CORPPensacola, FL | 3057083 | 1 |
| OBR TRANSPORT LLCClermont, FL | 1192314 | 1 |
| OC ORTIZ INCWaldo, FL | 3758095 | 1 |
| OCEAN MOVING & STORAGE CORPHollywood, FL | 1684609 | 1 |
| ODI TRANSPORTATION LLCCrestview, FL | 3866384 | 5 |
| ODORIZATION SOLUTIONS INCPensacola, FL | 1753507 | 12 |
| OEL EXPRESS INCHomestead, FL | 3800180 | 2 |
| OFFICE FURNITURE 4 LESS LLCOcala, FL | 4030937 | 1 |
| OFN LLCSarasota, FL | 7704353 | 1 |
| OGBESA TRUCKING INCOrlando, FL | 3336102 | 1 |
| OHL INTERNATIONAL LLCOviedo, FL | 3425036 | 3 |
| OJ THREE TRANSPORT CORPHialeah, FL | 2994011 | 1 |
| OK LOGISTICS CORPLake Placid, FL | 2976660 | 1 |
| OKEEFE TRUCKING LLCCape Coral, FL | 1167774 | 1 |
| OKELLY TRUCKING LLCSaint Cloud, FL | 3258617 | 1 |
| OKH EXPRESS CORPWinter Park, FL | 3106844 | 7 |
| OLDCASTLE LAWN & GARDEN INCLakeland, FL | 339115 | 91 |
| OLGA SEPULVEDADavenport, FL | 1396369 | 1 |
| OLIBRICE FAMILY TRANSPORTATION LLCLake Park, FL | 4415010 | 2 |
| OLIMA SERVICES LLCLehigh Acres, FL | 3711412 | 1 |
| OLIVARES TRUCKING INCPlant City, FL | 1530310 | 3 |
| OLVINSITO TRANSPORT INCKissimmee, FL | 2955958 | 2 |
| OMEGA EXPRESS TRUCKING LLCWest Palm Beach, FL | 2467830 | 2 |
| OMEGA WORLD TRADE LLCMiami, FL | 1734788 | 2 |
| OMNI GROUP US CORPHollywood, FL | 4198340 | 1 |
| OMPAF CARRIER INCMiami, FL | 2595672 | 10 |
| OMRA TRANSPORT INCSpring Hill, FL | 3558496 | 1 |
| OMV LOGISTICS LLCTampa, FL | 4205068 | 1 |
| ON THE CLOCK TRANSPORT CORPHomestead, FL | 3856969 | 1 |
| ON THE WAY YRM LLCLoxahatchee, FL | 4355224 | 1 |
| ON TIME TRUCKING CORPORATIONRoyal Palm Beach, FL | 2932662 | 2 |
| ONA LOGISTICS CORPMiami, FL | 3508448 | 1 |
| ONE FAM VENDING AND SERVICES INCMiami Gardens, FL | 4124148 | 1 |
| ONE FINE LINE LLCVenice, FL | 3358485 | 1 |
| ONE MAN TRUCKING LLCMiami, FL | 4210779 | 1 |
| ONE STOP INBOND CARRIERMiami, FL | 877787 | 1 |
| ONE WAY TRANSIT SYSTEM TRUCKING LLCMiami Gardens, FL | 3393498 | 3 |
| ONPOINT TRANSPORT SERVICES LLCTampa, FL | 4080431 | 3 |
| ONTIME EXPEDITE LLCSaint Johns, FL | 4045472 | 22 |
| OPTIMUS CARRIER SERVICES INCMalabar, FL | 3467979 | 10 |
| OPUS SOUTH CORPORATIONTampa, FL | 421106 | 1 |
| ORAINAS TRANSPORTATION LLCSun City Center, FL | 4373971 | 2 |
| ORAMA AND SON INCMiami, FL | 1981390 | 1 |
| ORANGE GATOR TRANSPORT, INC.Orlando, FL | 8576898 | 2 |
| ORION MARINE CONSTRUCTION INCTampa, FL | 16060 | 11 |
| ORISHA INCHialeah, FL | 2873595 | 1 |
| ORLANDO DRUM COOrlando, FL | 339290 | 6 |
| ORLANDO FRAME DECOR INCApopka, FL | 3201707 | 1 |
| ORLANDO TRUCK DRIVING ACADEMYSaint Cloud, FL | 4144529 | 1 |
| OSCAR M SOLARTEHollywood, FL | 1038854 | 1 |
| OSCAR MENESES LLCOrlando, FL | 4204645 | 1 |
| OSMANI POMPASt Petersburg, FL | 2346779 | 1 |
| OSMANY PEREZWest Palm Beach, FL | 1272875 | 1 |
| OSTAR EXPRESS LLCKissimmee, FL | 3462149 | 4 |
| OSVALDO VELEZHialeah, FL | 1523553 | 1 |
| OTL LOGISTICS INCNw Lake Placid, FL | 1433610 | 1 |
| OTM DEVELOPMENT LLCTallahassee, FL | 3894712 | 4 |
| OTTO PAULS TRANSPORT & SERVICE INCSaint Johns, FL | 2563368 | 1 |
| OUT THE BOX LOGISTICS LLCPalm Bay, FL | 4315068 | 1 |
| OUTDOOR DEPOT INCJacksonville, FL | 1324821 | 13 |
| OUTERBANKS TRANSPORT LLCPembroke Pines, FL | 4438403 | 1 |
| OVERLAND TRANSPORT LLCHialeah, FL | 4213751 | 7 |
| OVERNIGHT AUTO TRANSPORT INCMiami, FL | 2635612 | 2 |
| OVERSIZE SOLUTIONS LLCCantonment, FL | 4300944 | 1 |
| OVERSTREET EXPRESS LLCJacksonville, FL | 4106491 | 1 |
| OWEN OPERATIONS LLCClearwater, FL | 4558677 | 1 |
| OWENS DISTRIBUTORS INCSanford, FL | 875896 | 10 |
| OWNER BUSINESS INVESTMENTS LLCLake Worth, FL | 4285305 | 4 |
| OX TRUCKING LLCMiami, FL | 4373948 | 1 |
| OYGM EXPRESS INCLehigh Acres, FL | 4169169 | 1 |
| OYUELA TRUCKING LLCMiami, FL | 4134350 | 5 |
| OZZIECO INCMiddleburg, FL | 4016602 | 1 |